Antibody Discovery
& Optimization
In vivo and in vitro workflows in a single accountable path to nomination
Advance programs from concept to candidate selection using Mosaic’s antibody discovery and optimization services, integrating in vivo and in vitro workflows. Mosaic integrates in vivo antibody discovery with proprietary in vitro display platforms and function-first analytics to generate, optimize, and triage leads that are both biologically relevant and developable.
Our approach combines immunization strategies across multiple species and platforms with de novo in vitro discovery, library-based optimization, and early developability screening to reduce downstream risk and accelerate confident decision-making.
Candidate antibodies are frequently evaluated using functional cell-based assays to confirm activity and downstream signaling.
Antibody Discovery Platform
Integrated Discovery Engine
Integrate in vivo immunization with in vitro discovery and optimization workflows to generate and refine functional, developable binders across diverse epitopes.
Proprietary Naïve Libraries
Fab and VHH libraries designed for formatting flexibility and downstream optimization, including common light chain strategies for bispecific development.
NGS-Driven Insights
Repertoire mining to profile V(D)J usage, CDR3 diversity, clonal lineages, and somatic hypermutation to inform hit triage and engineering.
Custom Library Optimization
CDR shuffling, saturation mutagenesis, and structure-guided designs to improve affinity, specificity, and stability.
Function-First Analytics
Early developability screening, binding kinetics, epitope mapping, and cell-based functional assays to derisk candidates before nomination.
In Vivo Discovery
Immunization campaigns in:
- Humanized/transgenic mice (Atlas™ FHD)
- Wild-type strains
- Hyperimmune models
- Camelids for VHH/nanobody discovery
Antibody recovery via:
- Single B cell workflows
- Hybridoma generation
- Immune library construction
In Vitro Discovery & Optimization
High-diversity naive Fab and VHH libraries for antibody discovery
Phage display to explore epitope breadth and functional diversity
Yeast display for both naïve discovery and library-based optimization
Enabling affinity maturation, specificity refinement, and liability reduction.
Antibody Analytics
& Developability Screening
Repertoire Mining
Identify convergent clonotypes, broaden epitope bins, and trace affinity maturation lineages.
Post-Hoc Hit Expansion
Recover low-frequency family members around validated leads to expand therapeutic options.
IP Strengthening
Capture near-neighbor sequences to expand freedom to operate and build robust patent positions.
Developability Triage
Early computational and experimental filters for aggregation, PTM liabilities, and expression risk.
Antibody Discovery Timelines
At Mosaic, we combine scientific expertise, strategic planning, and collaborative execution to advance therapeutic programs efficiently and effectively.
Antibody Discovery Deliverables
Rank-ordered antibody leads
Binding kinetics and affinity data
Epitope mapping and binning
Specificity and cross-reactivity profiling
Expression and stability data
Clear decision path to candidate selection
Developability metrics
Explore Mosaic’s full suite of biologics discovery solutions supporting antibody discovery, engineering, and downstream characterization.
Get Started
Start a conversation with our scientific team about your biologic discovery program.
Explore Other Discovery Solutions
Complementary capabilities supporting biologics discovery, engineering, and characterization.
Protein Design & Engineering
Cell Based Assays
Protein Sciences & Analytics
